Table 1.

Oligonucleotide primers used in RT-PCR analysis for human IGF-I, IGF-II, and β-actin mRNA expression in DU-145 human androgen-independent prostate tumors

mRNA	Primers	Sequences (5′ … 3′)	Position	Target size	Ref.
IGF-I	Sense	ACATCTCCCATCTCTCTGGATTTCCTTTTGC	85–116	514 bp	29
	Antisense	CCCTCTACTTGCGTTCTTCAAATGTACTTCC	567–598
IGF-II	Sense	AGTCGATGCTGGTGCTTCTCACCTTCTTGGC	270–300	538 bp	30
	Antisense	TGCGGCAGTTTTGCTCACTTCCGATTGCTGG	776–807
β-actin	Sense	ATCTGGCACCACACCTTCTACAATGAGCTGCG	294–326	518 bp	31
	Antisense	GCAGCGGAACCGCTCACCGCCAATGGTGAT	782–812