Table 1.

2-5A-antisense targeting the RSV genomic RNA

Antisense domain		5′-AAA AAT GGG GCA AAT AA-3′	Mismatches†
Sequence number	Potential sense target*	Targeted sequence	Terminal	Internal
1	3′ leader/NS1 start	3′-UUU UUA CCC CGU UUA UU-5′	0	0
2	NS1/NS2 gene start	3′-UUU UUA CCC CGU UUA UU-5′	0	0
3	N/P gene start	3′-UUU UUA CCC CGU UUA UU-5′	0	0
4	F/M2 gene start	3′-UUU UGA CCC CGU UUA UA-5′	1	1
5	NS2/N gene start	3′-CUU CUA CCC CGU UUA UG-5′	2	1
6	M/SH gene start	3′-UGU GUA CCC CGU UUA UU-5′	0	2
7	G/F gene start	3′-U UG UGA CCC CGU UUA UU-5′	0	2
8	P/M gene start	3′-U UC CCA CCC CGU UUA UA-5′	1	3
9	SH/G gene start	3′-U UG UAA CCC CGU UUA CG-5′	1	3
10	L gene start	3′-U CA ACA CCC UGU UUU AC-5′	1	7

^*Gene-end–intergenic–gene-start sequences are described by naming the gene end, with a “/” representing the intergenic sequence, followed by naming the gene start. 

^†Underlined nucleotides are mismatches to the 2-5A-chimera antisense domain. Oligonucleotide target sequences are listed in theoretical order of hybridization efficiency to the antisense domain sequence. Mismatches defined as “internal” or “terminal” refer to the annealing antisense oligonucleotide.