Table 1 Toll‐like receptor (TLR)2 and TLR4 restriction fragment length polymorphism analysis*.
| Gene polymorphism | Restriction enzyme | Restriction fragment length (bp) | Primer sequence |
|---|---|---|---|
| TLR2 Arg753Gln | Msp 1 | Wild type (G allele): 104 + 25 | F: cattccccagcgcttctgcaagctcc |
| Arg753Gln (A allele): 129 | R: ggaacctaggactttatcgcagctc | ||
| TLR4 Asp299Gly | Nco 1 | Wild type (A allele): 188 | F: agcatacttagactactacctccatg |
| Asp299Gly (G allele): 168 + 20 | R: gagagatttgagtttcaatgtggg | ||
| TLR4 Thr399Ile | Hinf 1 | Wild type (C allele): 124 | F: ggttgctgttctcaaagtgattttgggagaa |
| Thr399Ile (T allele): 98 +26 | R: ggaaatccagatgttctagttgttctaagcc | ||
| Gene polymorphism | Allele | Controls n = 86 (TLR2) n = 85 (TLR4) | Idiopathic bronchiectasis n = 94 | Odds ratio (95% CI) | p Value |
|---|---|---|---|---|---|
| TLR2 Arg753Gln | G allele | 169 (98.2) | 180 (95.7) | — | — |
| A allele | 3 (1.8) | 8 (4.3) | 2.50 (0.65 to 9.59) | NS | |
| TLR4 Asp299Gly | A allele | 162 (95.3) | 177 (94.1) | — | — |
| G allele | 8 (4.7) | 11 (5.9) | 1.26 (0.49 to 3.21) | NS | |
| TLR4 Thr399Ile | C allele | 163 (95.9) | 177 (94.1) | — | — |
| T allele | 7 (4.1) | 11 (5.9) | 1.45 (0.56 to 3.82) | NS |
F, forward; R, reverse; TLR, toll‐like receptor.
The table shows the frequencies of TLR2 and TLR4 polymorphisms in patients with idiopathic bronchiectasis and in controls.
*As described previously by Folwaczny et al.7