Figure 3.

(A) RNase protection assay for CCR5 mRNA in CD4⁺ lymphocytes of four healthy donors (1–4) in the absence and presence of IL-4. Cellular transcripts glyceraldehyde-3-phosphate dehydrogenase and L32 were used as internal controls. Each RNA sample was analyzed in two independent experiments with the same results. (B) Quantification of CCR5 mRNA expression in A. The amount of RNA per sample was normalized to the control glyceraldehyde-3-phosphate dehydrogenase by using the imagequant program. Similar results were obtained with L32 as internal standard. (C) RNase protection assay for CCR5 mRNA in 14-day-old attached macrophages from a healthy donor in the absence of any cytokines (−) and in the presence of 3 ng/ml of either IL-4, IL-5, or IL-10, as indicated. (D) Quantification of CCR5 mRNA expression in C.