Table 1.
FACS analysis of CCR5 and CXCR4 expression in human PBMC of different healthy donors
| Donor | CCR5
|
CXCR4
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| IL-4 (−)
|
IL-4 (+)
|
IL-4 (−)
|
IL-4 (+)
|
||||||
| % Positive cells | MFI | % Positive cells | MFI | Donor | % Positive cells | MFI | % Positive cells | MFI | |
| 1 | 22 | 135 | 10 | 164 | 1 | 89 | 183 | 84 | 256 |
| 2 | 29 | 146 | 17 | 202 | 2 | 94 | 219 | 92 | 292 |
| 3 | 27 | 63 | 10 | 62 | 3 | 88 | 159 | 96 | 226 |
| 4 | 46 | 71 | 21 | 128 | 4 | 82 | 58 | 85 | 91 |
| 5 | 35 | 42 | 15 | 50 | |||||
| 6 | 55 | 49 | 26 | 57 | |||||
The assays were performed as in Fig. 2A. The mouse mAb 12G5(45) was used for CXCR4 detection. MFI, mean fluorescence intensity. The values shown were obtained from the complete phytohemagglutinen-stimulated PBMC samples.