Table 1 Oligonucleotide used as primers for real‐time RT‐PCR.
| Gene name | Oligonucleotide | Sequence | Position | Amplicon |
|---|---|---|---|---|
| Agt | Forward primer | GTGGAGGTCCTCGTCTTCCA | 1239–1258 | 108 |
| Reverse primer | GTTGTAGGATCCCCGAATTTCC | 1346–1325 | ||
| Ace | Forward primer | CGGTTTTCATGAGGCTATTGGA | 3080–3101 | 102 |
| Reverse primer | TCGTAGCCACTGCCCTCACT | 3181–3162 | ||
| Ace2 | Forward primer | ACCCTTCTTACATCAGCCCTACTG | 755–778 | 74 |
| Reverse primer | TGTCCAAAACCTACCCCACATAT | 828–806 | ||
| Agtr1 | Forward primer | GAAGCCAGAGGACCATTTGG | 1260–1279 | 101 |
| Reverse primer | CACTGAGTGCTTTCTCTGCTTCA | 1360–1338 | ||
| Agtr2 | Forward primer | GCCAACATTTTATTTCCGAGATG | 528–550 | 81 |
| Reverse primer | TTCTCAGGTGGGAAAGCCATA | 608–588 |
Agt, angiotensinogen; Ace, angiotensin‐converting enzyme; Ace2, angiotensin‐ converting enzyme 2; Agtr1, type 1 angiotensin II receptor; Agtr2, type 2 angiotensin II receptor.