Table 1.

Summary of the on-line digestion experiments for cytochrome C. The experiments were conducted at pH 8.5 and 37°C (n = 3)

capillary ID (μm)	reactor volume (μL)	amount injected		digestion time (s)	% undigested protein (SD)
		(μg mL−1)	(pmol)
75	1	248	20	60	65.9 (4.8)
75	1	124	10	60	35.8 (3.4
75	1	12.4	10	60	23.6 (1.3)
50	1	248	20	60	14.0 (1.9)
50	1	124	10	60	2.3 (2.0)
50	2.75	248	20	165	0
50	2.75	124	10	165	0
50	2.75	12.4	10	165	0

The experiments were conducted at pH 8.5 and 37 °C (n = 3)