Table 3.

Peptide fragments observed by MS in the on-line digestion of cytochrome C and myoglobin The experiments were conducted at pH 8.5 and 37 °C at a flow rate of 1 μL min⁻¹ using a 2.75-μL trypsin reactor

Peptide sequence	Theoretical m/z	Experimental m/z	Position	Missed cleavage
Cytochrome C (sequence coverage 87%)
1....IFVQKCAQCHTVEK	1632.8	816.9 (2+)	9–22	1
2....HKTGPNLHGLFGR	1432.8	717.1 (2+)	26–38	1
3....TGPNLHGLFGR	1167.6	584.6 (2+)	28–38	0
4....TGPNLHGLFGRK	1295.7	648.6 (2+)	28–39	1
5....KTGQAPGFTYTDANK	1597.8	533.4 (3+)	39–53	1
6....TGQAPGFTYTDANK	1469.7	735.6 (2+)	40–53	0
7....TGQAPGFTYTDANKNK	1711.8	571.4 (3+)	40–55	1
8....GITWKEETLMEYLENPKK	2208.1	736.8 (3+)	56–73	2
9....EETLMEYLENPK	1494.7	748.0 (2+)	61–72	0
10 EETLMEYLENPKK	1622.8	812.1 (2+)	61–73	1
11...YIPGTK	678.4	678.1 (1+)	74–79	0
12...MIFAGIKK	906.5	454.0 (2+)	80–87	1
13...KTEREDLIAYLKK	1477.8	739.6 (2+)	88–99	2
14...TEREDLIAYLKK	1349.7	675.5 (2+)	89–99	1
15...EDLIAYLK	963.5	482.6 (2+)	92–99	0
16...EDLIAYLKK	1091.6	546.6 (2+)	92–100	1
Myoglobin (sequence coverage 88%)
1....GLSDGEWQQVLNVWGK	1814.9	908.7 (2+)	1–16	0
2....VEADIAGHGQEVLIR	1605.8	803.6 (2+)	17–31	0
3....VEADIAGHGQEVLIR	1605.8	536.1 (3+)	17–31	0
4....LFTGHPETLEK	1270.7	636.0 (2+)	32–42	0
5....HLKTEAEMK	1085.6	543.5 (2+)	48–56	1
6....HGTVVLTALGGILK	1377.8	689.7 (2+)	64–77	0
7....HGTVVLTALGGILKK	1505.9	502.7 (3+)	64–78	1
8....KKGHHEAELKPLAQSHATK	2109.1	703.7 (3+)	78–96	2
9....KGHHEAELKPLAQSHATK	1981.0	661.1 (3+)	79–96	1
10 GHHEAELKPLAQSHATK	1853.0	618.8 (3+)	80–96	0
11...YLEFISDAIIHVLHSK	1884.0	628.8 (3+)	103–118	0
12...HPGNFGADAQGAMTK	1500.7	751.5 (2+)	119–133	0
13...ALELFRNDIAAK	1359.8	680.6 (2+)	134–145	1
14...YKELGFQG	940.5	471.0 (2+)	146–153	1