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. 2007 Dec 19;2(12):e1315. doi: 10.1371/journal.pone.0001315

Figure 8. In vitro recombination catalyzed by wild type IntI1 in presence of double- or single-stranded substrates.

Figure 8

Reactions were performed for 90 min in the presence of purified enzyme (5 pmoles), 0.1 pmoles of linear radiolabeled double-stranded (ds) or single-stranded (bottom strand: ss bot, or top strand: ss top) recombination sites attI1 or attC and 0.1 pmoles of pGEM-T-attI1 or pGEM-T-attC under standard conditions described in materials and methods section. Products were loaded on 1% agarose gel and autoradiographied. F: free recombination sites, RP: recombination products.