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. 2007 Dec 19;2(12):e1315. doi: 10.1371/journal.pone.0001315

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Dubois et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Recombination reactions were performed in the presence of 2 pmoles purified IntI1, 0.1 pmoles free attI1^* and 0.1 pmoles pGEM-T-attI1 and different concentrations of MgCl₂, MnCl₂ NaCl and detergent as indicated. Recombination products were quantified with DNAJ software and are shown on the graphs as the percentage of recombinant product versus the total substrate.