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. Author manuscript; available in PMC: 2007 Dec 6.
Published in final edited form as: J Am Chem Soc. 2005 Dec 14;127(49):17364–17376. doi: 10.1021/ja0554054

Figure 2.

Figure 2.

Determination of MMOH-MMOR-Fd(1-107) binding constants by isothermal titration calorimetry. (A) Data recorded for complex formation between MMOH (12.1 μM in the calorimeter cell) and MMOR-Fd(1-107) (453 μM in a 250-μL injection syringe) at 4.2 °C. Each heat pulse corresponds to a 10-μL injection into the 1.430-mL sample cell. (B) Integrated enthalpy data (circles) fit with an interacting sites binding model after correction of the MMOH concentration for the nonbinding fraction of hydroxylase (solid line).