Figure 6.

(A) The effect of perfusion of recombinant adenovirus containing either wild-type OB-Rb cDNA or β-gal cDNA into pancreata from fa/fa ZDF rats on wild-type OB-Rb mRNA and protein in islets isolated immediately after perfusion and cultured for 3 days. Both OB-Rb mRNA and protein are intensely overexpressed. (B) Effect of overexpression of OB-Rb on PPARα mRNA of cultured fa/fa islets in the absence or presence of 20 ng/ml of recombinant leptin. Leptin was added 24 hr after the start of the 3-day culture period. Bars represent mean (±SEM) of three experiments. ∗, P < 0.01 vs. 0 ng/ml of leptin.