Figure 1.

[³⁵S]Methionine-labeled proteins expressed in human orbital fibroblasts and separated by giant two-dimensional gel electrophoresis. Shown is a typical autoradiogram of human orbital fibroblast proteins. Confluent fibroblasts from a strain derived from a patient with TAO were incubated in the presence of leukoregulin (1 unit/ml) for 15 h and metabolically labeled for 1 h. Cultures were terminated by addition of a 9.5 M urea lysis buffer and proteins separate electrophoretically as described. The symbols locate the proteins of interest: the squares locate the two orbital-specific (OS) proteins, the rectangle the 72/74 cyclooxygenase 2 protein (PGHS-2) isoforms, the ovals the CRPs, and the arrows the five ANPs.