Figure 3.

ANPs in fibroblasts from three anatomical regions. Portions of typical autoradiograms locating the ANPs are shown. These autoradiograms are generated from three fibroblast strains derived from a single patient with severe TAO. Confluent cultures were treated with nothing or with leukoregulin (1 unit/ml) for 15 hr and were processed as described in Experimental Procedures. This figure demonstrates the inductions of five ANPs in orbital and pretibial dermal fibroblasts and a sixth spot that is induced massively by leukoregulin in orbital and pretibial fibroblasts and only slightly in fibroblasts from the abdominal wall.