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Southern blot analysis of H. influenzae transposon mutants. Genomic DNA was isolated from 16 individual mutants and was digested with AseI, which cleaves once within magellan1. Digested DNA was subjected to agarose gel electrophoresis, was transferred to nitrocellulose, and was hybridized with a probe composed solely of magellan1 minitransposon-derived DNA.
