Figure 3.

Genetic footprinting of H. influenzae mutant pools. Genetic footprinting was carried out by using a Himar1-specific primer and a chromosomal primer. In a, the positions of molecular weight standards are indicated; other panels are labeled with locus names by HI number. In c and d, cells were selected on BXV, MIc, or BXV containing trimethoprim (“Tri”). In f, in vitro mutagenesis of a chromosomal fragment that included the secA gene was performed, and the mutagenized DNA was transformed into both wild-type H. influenzae and an H. influenzae strain containing pSecA.