Table 1.

S. pneumoniae essential genes

S. pneumoniae ORF*	Position†	Essential‡	Similarity (gap-blast E-value)
Conserved hypothetical	840–2174	No	Archaeoglobus fulgidus, hypothetical protein, AF0170, (1e–47)
Unknown	3051–3866	No	None
rbfA	4109–4459	Yes	Bacillus subtilis ribosome-: binding factor A, P32731, (4e–20)
IF-2	4710–7586	Yes	H. influenzae translation, initiation factor IF-2, P44323, (1e–153)
L7AE	7603–7902	Yes	Enterococcus faecium, probable ribosomal protein in L7AE family, P55768, (6e–23)
nusA	8210–9346	Yes	B. subtilis NusA, Z99112, (3e–96)
p15A	9390–9860	No	B. subtilis P15A homolog, unknown function P32726, (2e–27)
ytmQ	9995–10630	No	B. subtilis YtmQ, unknown function, Z99119, (5e–73)

PCR Primers used to amplify the 11,266 bp corresponding to contig 4151 of TIGR S. pneumoniae genomic sequence release 112197 and are Forward 5′-CTTTCTGTAAAATGTGGGATTCAA-3′ and Reverse 5′-AATTATTATGGAGTCGTCGTTTGG-3′. 

^*S. pneumoniae orf designations are based on matches giving the highest gap-blast score. 

^†Positions are given with respect to the first base of the Forward primer. 

^‡Essential regions as defined in the text.