Figure 4.

Solubilization of bovine taste receptors. Trypsin assays to monitor G-protein activation. (A) Native transducin was activated by denatonium and bovine taste membranes in the presence of 1% n-octyl glucoside (OG) (Left); transducin was similarly activated by the OG solubilized taste membrane supernatant (Right). (B) In vitro translated gustducin was activated by denatonium and bovine taste membranes (Left); gustducin was similarly activated by the OG solubilized taste membrane supernatant (Middle). The receptor interaction-defective mutant of gustducin (G352P-gustducin) was not activated by denatonium plus solubilized taste membranes (Right). Den, 5 mM denatonium benzoate.