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. 2006 Jan 23;203(1):47–52. doi: 10.1084/jem.20051886

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Phenotypic analysis of CD3γ^−/− and γδTCR Tg CD3γ^−/− mice. (A) Comparison of γδTCR surface levels on DN thymocytes and splenocytes from CD3γ^−/− (bold line) and CD3γ^+/+ (gray line) mice (top) and from γδTCR Tg CD3γ^−/− (bold line) and γδTCR Tg CD3γ^+/+ (gray line) mice (bottom). DN thymocytes and splenocytes are defined as CD4⁻CD8⁻CD19⁻. Staining with a hamster isotype control is also shown (shaded histogram). (B) Number of γδTCR⁺ CD4⁻CD8⁻CD19⁻ cells in the thymus and spleen of CD3γ^+/+ (n = 4) and CD3γ^−/− (n = 6) mice. Bars represent means ± standard deviation. (C) Comparison of αβTCR surface levels on CD4⁺ thymocytes and splenocytes from CD3γ^+/+ (gray line) and CD3γ^−/− (bold line) mice. Staining with a hamster isotype control is also shown (shaded histogram).