Figure 5.
In vitro measurements of peak sarcomere shortening values (μm) (A), normalized maximum contraction velocity (−dL/dt) (B), normalized maximum relaxation velocity (+dL/dt) (C), time to maximum contraction velocity (ms) (D), and time to maximum relaxation velocity (ms) (E) 24 h after CLP or sham operation. Anti-C5a or nonspecific IgG was injected at the time of operation. Using IonWizard Software and the IonOptix Acquisition System, 10–15 sarcomeres were included in a rectangle-shaped region of interest and contractions were recorded for 75 s, averaged, and analyzed. Each bar reflects several n = 5 rats; total number of analyzed cells, n = 649. *, P < 0.001 (ANOVA).