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. 2006 Jan 23;203(1):41–46. doi: 10.1084/jem.20051512

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — IFN-α augments induction of APOBEC3 family members. (A) RNA was isolated from macrophages (two donors shown) untreated or treated with 10 ng/ml IFN-α for 4 h and evaluated by RT-PCR with primers corresponding to each of the indicated APOBEC3 genes and PKR (30 cycles). GAPDH was used as a parallel control. (B) Monocytes were transfected with APOBEC3G siRNA, cultured for 6 d, stimulated with IFN-α for 4 h, and APOBEC3G and PKR expression was monitored by RT-PCR in comparison with the GAPDH control. (C) Western blot for APOBEC3G protein 6 d after monocyte transfection with siRNA or control RNA, or electroporation only control (EPC), and treatment with or without IFN-α for 20 h. Representative experiment, n = 3.