Figure 2.

DUSP1 is induced after injection of LPS and controls survival and cytokine production. (A) Expression levels of DUSP1 in the spleens of WT mice were determined at the indicated times after injection of 10 mg/kg LPS i.p. by Northern blot analysis of 10 μg total RNA. Data shown are from individual mice (n = 3–4) from one representative experiment. (B) Survival of WT (n = 15) and DUSP1^−/− mice (n = 13) after i.p. injection of 25 mg/kg LPS. Data are pooled from two experiments. (C and D) Serum levels of cytokines after LPS injection (10 mg/kg). Data shown are the mean and standard deviation (n = 3–4 mice per data point) from representative experiments. Two data points marked by asterisks were beyond the maximum of the standard curve, and the upper limits of the dynamic range of the assay are shown. (C) TNF-α and IL-6 in WT and DUSP1^−/− mice on a mixed 129Sv × Bl/6 (left) or pure 129Sv and C3H (right) background (6 h after LPS). (D) Comparison of the effect of deficiency in DUSP1 or IL-10 on serum levels of TNF-α and IL-12p40.