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. 2006 Jan 23;203(1):15–20. doi: 10.1084/jem.20051753

Figure 3.

Figure 3.

Genome-wide analysis of LPS-induced gene expression in the spleens of WT and DUSP1−/− mice. (A) Total splenic RNA was prepared from control WT and DUSP1−/− mice 6 h after i.p. injection of 10 mg/kg LPS (n = 3 per group) and was processed for Affymetrix GeneChip analysis. Venn diagram comparing the numbers of genes significantly up-regulated in WT and DUSP1−/− mice by LPS (for criteria see Materials and methods). Z-score–normalized expression values of LPS-induced genes were subjected to hierarchical clustering analysis. Cluster A contains a group of genes expressed more strongly in the WT spleen. In contrast, cluster C represents a larger subset of LPS-induced genes up-regulated in the absence of DUSP1. Expression data of these 608 genes can be found in Table S1. (B) Differential control of the chemokines CCL3, CCL4, CXCl2, and CXCL10 by DUSP1. Confirmation of the microarray expression data (left: average and standard deviation of signal intensity values) by ELISA determination of serum levels (right: mean and standard deviation, n = 3–4). (C) Increased expression of IL-10 at the mRNA and protein level in DUSP1−/− mice. Northern data from two and three individual mice per condition and organ are shown, and ELISA data are the mean and standard deviation (n = 3–4) from a representative experiment.