Figure 3.
Isolation of LC by migration in vitro. (A) Morphological changes in LC during the course of transplantation illustrated using anti CD1a-FITC and DAPI counterstain. Bar, 20 μm. (B) Flow cytometry of migrant cells. LC are visible by scatter properties (left, oval region) and CD1a expression (right). CD3+ or CD14+ cells are <1%. The data are representative of 30 sex-matched transplants of whom 11 had active skin GVHD (clinical stages 1–2) at the time of biopsy. (C) Giemsa stain of epidermal migrants showing distinctive LC and keratinocyte morphology.