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. 2006 Jan 23;203(1):215–226. doi: 10.1084/jem.20051774

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — In 1.B4.B6 cells, the VH and Sμ regions have distinguishable histone Ac patterns. Anti-(α)H3Ac or αH4Ac histone antisera were used in ChIP assays performed on nuclei derived from 1.B4.B6 cells that were unstimulated (Unstim) or stimulated with LPS+CD40L (L+C) and LPS+CD40L+IL-4 (L+C+IL4) for 48 h. The results from four immunoprecipitated samples derived from three independent experiments are shown with standard deviations. Samples were analyzed in duplicate then averaged. All ChIP PCR values were normalized against ChIP for the Gapdh locus and input DNA in the same sample.