Figure 4.
(A) RU-486 prevents Dex-induced apoptosis of PD1.6 cells. PD1.6 cells were incubated with various concentrations of Dex in the absence or presence of 5 μM RU-486 for 20 h. Percentage of apoptosis was determined as in Fig. 1 A. (B) RU-486 induces mitochondrial translocation of GR in PD1.6 cells. Cells were incubated in the absence or presence of 5 μM RU-486 and/or 100 nM Dex for 2 h followed by subcellular fractionation and Western blotting.