Figure 6.

LIT1-null L. amazonensis differentiate normally into the intracellular mammalian stages. BMmø were infected with wild-type or Δlit1 parasites (A, axenic amastigotes; B, metacyclic promastigotes) for 1 h, followed by washes, further incubation for 24 h, fixation, permeabilization, and immunofluorescence with antibodies against the amastigote-specific antigen P4 (reference 29). (A and B, left) Phase contrast; (A and B, middle and right) fluorescence microscopy. Anti-P4 (green) and host and parasite DNA (blue) are shown.