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. 2006 Oct 2;203(10):2305–2314. doi: 10.1084/jem.20060411

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — Normal self-renewal/homeostatic proliferation of Blimp-1–deficient B-1 cells. Equal numbers of total PerC cells from control and CD19^Cre/+prdm1^Flox/Flox mice were transferred i.p. to Rag1-deficient hosts. 6–8 wk after transfer, PerC cells were harvested from recipient mice and the efficiency of B-1 cell reconstitution was determined by dividing the number of B-1 cells recovered by the number transferred. (A) Representative flow cytometry plots for anti-IgM– and Mac-1–stained PerC cells from Rag1^−/− recipient mice either untreated (left) or receiving WT PerC cells (middle) or CD19^Cre/+prdm1^Flox/Flox PerC cells (right) are shown. IgM⁺Mac1⁺ B-1 population is shown. (B) Bar graph showing the mean and SEM of the percentage of WT (filled bar) and CD19^Cre/+Prdm1^Flox/Flox (open bar) B-1 cells recovered for five WT and six CD19^Cre/+prdm1^Flox/Flox samples. (C) Total PerC cells harvested from mice in B.