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. 2006 Oct 2;203(10):2293–2303. doi: 10.1084/jem.20060921

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Copyright © 2006, The Rockefeller University Press

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Figure 6. — Selection and maturation of iNKT cells is compromised in the thymus of Sandhoff and GM1 gangliosidosis mice. Four-color flow cytometry was performed on thymocytes from Sandhoff, GM1 gangliosidosis, and age-matched control mice. Cells were stained with NK1.1 and CD44 antibodies, a pan–TCR Vβ antibody, and an α-GalCer/CD1d tetramer. The maturation phenotype was analyzed by gating on CD3⁺/tetramer⁺ cells. (A) The percentage of thymic iNKT cells of the immature CD44⁻/NK1.1⁻, semimature CD44⁺/NK1.1⁻, or mature CD44⁺/NK1.1⁺ phenotype as a percentage of α-GalCer/CD1d tetramer⁺/Vβ⁺ cells. (B) Total number of iNKT cells at three different stages of maturation (CD44⁻/NK1.1⁻, CD44⁺/NK1.1⁻, and CD44⁺/NK1.1⁺) from homozygous Sandhoff or GM1 gangliosidosis mice and controls are shown. Data represent one of two independent experiments (mean ± SE; n = 3–5 mice/group).