Table II.
Percentages of APCs within different disease models
| Spleen
|
Thymus
|
Liver
|
||||
|---|---|---|---|---|---|---|
| Macrophage | DC | Macrophage | DC | Macrophage | DC | |
| C57BL/6 | 8.2 ± 0.5 | 2.5 ± 0.1 | 0.71 ± 0.04 | 0.51 ± 0.03 | 11.6 ± 1.9 | 3.1 ± 0.4 |
| Sandhoff | a11.3 ± 0.8 | a1.6 ± 0.2 | 0.74 ± 0.1 | 0.6 ± 0.02 | 8.7 ± 1 | 3.6 ± 0.5 |
| Tay-Sachs | 9.2 ± 1.2 | a1.7 ± 0.1 | 0.66 ± 0.03 | a0.64 ± 0.03 | 8.1 ± 0.8 | a1.5 ± 0.4 |
| GM1 gangliosidosis | a10.2 ± 0.4 | a1.9 ± 0.1 | 0.95 ± 0.1 | 0.61 ± 0.05 | 9.7 ± 1 | 2.8 ± 0.4 |
| Fabry | a10.5 ± 0.6 | 2.3 ± 1.1 | a0.54 ± 0.02 | 0.61 ± 0.05 | 14 ± 2.7 | 3.8 ± 0.5 |
| NPC1+/+ | 4.9 ± 0.2 | 1.01 ± 0.02 | 0.44 ± 0.02 | 0.61 ± 0.02 | 12.5 ± 2.02 | 1.8 ± 0.2 |
| NPC1 | a4 ± 0.1 | 1.17 ± 0.07 | a0.55 ± 0.02 | a1.1 ± 0.13 | 9.22 ± 0.24 | a3.26 ± 0.005 |
Cell suspensions from the various tissues were stained with antibodies against CD11c and CD68 to detect DCs and macrophages, respectively.
a
Statistical significance between GSL storage disease and controls using the t test (P ≤ 0.05).