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. 2006 Oct 30;203(11):2413–2418. doi: 10.1084/jem.20061166

Figure 1.

Figure 1.

TRAF3 deficiency results in constitutive processing of p100 in MEFs. (A) WT and TRAF3 / MEFs were stimulated with an anti-LTβR antibody for the indicated times. Processing of p100 to p52 in whole cell extract was detected by immunoblot. Total β actin is shown as a loading control. (B) Whole cell extracts from WT and TRAF3 / MEFs reconstituted with either pBABEpuro-TAP or pBABEpuro-TAP-TRAF3 were assessed for basal p100 processing to p52 by immunoblot. Reconstitution of TRAF3 expression in TRAF3 / MEFs was confirmed by immunoblot. Total β actin is shown as a loading control. *, endogenous TRAF3.