Table I.
Analysis of CSR on the t(14;18)+ translocated allele in peripheral blood from HI
Sample | BCL2/JH
a
|
BCL2/Sμ
|
BCL2/Sγ
|
|||||||
---|---|---|---|---|---|---|---|---|---|---|
Detection | Ratio | Frequency | Detection | Ratio | Associated JH | Detection | Ratio | Associated JH | Isotype | |
99 | yes | 11:30 | 1.1 × 10−5 | no | 0:10 | - | yes | 1:5 | JH6* | γ1 |
102 | yes | 23:30 | 3.6 × 10−5 | yes | 1:5 | JH5 | yes | 1:5 | JH5 | γ1 |
103 | yes | 14:30 | 1.6 × 10−5 | yes | 1:10 | JH6b | no | 0:10 | - | - |
109 | yes | 12:30 | 1.3 × 10−5 | no | 0:10 | - | yes | 1:10 | JH? | γ2 |
120 | yes | 11:30 | 1.1 × 10−5 | no | 0:10 | - | yes | 2:10 | JH4* | γ1 |
127 | yes | 23:30 | 3.6 × 10−5 | no | 0:10 | - | yes | 4:10 | JH6* | γ2 |
JH6* | γ1 | |||||||||
Mean | 2.1 × 10−5 | |||||||||
Total | 6:6 | 2:6 | 2:55b | 5:6 | 9:50b |
Previously described in reference 8 Ratio, positive replicates:total replicates.
Cumulative occurrence of BCL2/Sμ junctions, as determined by the Pearson's χ2 test, was significantly lower than BCL2/Sγ junctions (P = 0.02).
BCL2/JH junction identical to the one previously found with the BCL2/JH PCR assay (8).