Figure 5.

The MPD in 12/15-LO–deficient mice is cell autonomous. (a) Increased splenic weight, (b) representative H &E-stained cryostat sections of spleens, and (c) cytospins of splenocytes from 8–10-wk-old wild-type mice injected via the tail vein with 0.5 × 10⁶ (Alox15 crisis) or 10⁷ (Alox15 chronic and C57BL/6) splenocytes or 2 × 10⁶ bone marrow cells, monitored every other day, and killed at 6 or 10 wk after transfer. Bar, 50 μm. (d) 2% agarose electrophoresis of PCR products for the neomycin cassette performed on DNA extracted from spleens taken from mice transplanted with B6 (B6 Trx) or Alox15 (ATrx) cells versus Alox15 tail DNA positive control. (e) Table summarizing transfer experiments performed as described in panel a. Splenic distortion was assessed blindly. All error bars represent means ± SD.