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. 2006 Oct 30;203(11):2529–2540. doi: 10.1084/jem.20061444

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — ICSBP defect and enhanced Bcl-2 expression in cells from Alox15 mice. (a) Immunoblot of nuclear extracts prepared from wild-type (B6), chronic Alox15 (Chr), and crisis Alox15 (Cri) splenocytes using antibodies specific for ICSBP and normalized to total protein, as confirmed by coomassie stain (loading). (b) Real-time PCR analysis of ICSBP mRNA expression levels in wild-type, chronic Alox15, and crisis Alox15 splenocytes. *, P = 0.0115 and 0.0284 compared with B6 and Chr, respectively. (c) Real-time PCR of sorted myeloid splenocytes isolated from C57BL/6 and chronic stage Alox15 mice. (d) Representative immunoblot of nuclear (above line) and cytoplasmic (below line) extracts prepared from sorted wild-type and Alox15 splenocytes for ICSBP, 12/15-LO, Bcl-2, and the loading controls histone 2b and total actin. The results are representative of three experiments. All error bars represent means ± SD.