Figure 1.

Role of IL-17 in development of allergic inflammation, as assessed in receptor IL-17R KO mice. OVA/alum-sensitized C57BL/6 mice (WT) and IL-17R KO (IL-17R^−/−) mice were challenged three times intranasally with OVA. 48 h after the third challenge, the BAL cells and eosinophils were counted (A and B), and lung tissue activities of EPO (C) and OVA-specific serum IgE concentrations (D) were determined. Mediastinal lymph nodes (MLNs) were removed from antigen-sensitized and challenged mice, restimulated in vitro with 50 μg/ml OVA, and analyzed for IL-5 production (E). In F, the cell composition of freshly excised MLNs was determined by FACS analysis. The percentages of CD3-, CD11b- and CD11c-positive cells are shown. The bars represent the mean ± SD (n = 8 animals per group). *, P ≤ 0.05; dotted line indicates basal levels.