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IL-17 inhibits IL-5, IL-13, and Rantes production in mediastinal lymph nodes (MLNs). MLN (A–D) and splenocyte (E–H) cultures were prepared from OVA-sensitized and challenged animals. Cells were treated with saline, 50 μg/ml OVA alone, IL-17 alone, or OVA plus 30 ng/ml IL-17 for 24 h. The concentrations of IL-5, IL-13, TARC, and Rantes were measured by ELISA. The results represent the mean ± SD of 8 animals per group. n.d., not detected. *, P ≤ 0.05; dotted line indicates basal levels.
