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. 2006 Nov 27;203(12):2703–2714. doi: 10.1084/jem.20051730

Figure 1.

Figure 1.

JAM-C is recruited to the junctions of HDMECs after stimulation by VEGF or histamine. (A) Immunofluorescence analysis followed by confocal microscopy of the interendothelial contacts of HDMECs. Representative stainings of HDMECs that were incubated in the absence (−) or presence of VEGF (1 h, 50 ng/ml) or histamine (1 h, 50 μM), showing the distribution of JAM-C and ZO-1. Double stained images were merged to analyze colocalization. (B) The surface versus total expression of JAM-C in nonpermeabilized versus permeabilized HDMECs, respectively, was analyzed by cell ELISA. HDMECs were pretreated without (−; open bars) or with VEGF (1 h, 50 ng/ml; filled bars) or histamine (1 h, 50 μM; gray bars) as indicated. JAM-C expression is shown as the percentage of control, defined as the total JAM-C expression in the absence of any stimulus. Data are mean ± SD (n = 3) of one experiment typical of three separate experiments. (C) Analysis of the interendothelial contacts of HAECs was performed as in A. Representative immunofluorescence of HAECs that were incubated in the absence (−) or presence of VEGF (1 h, 50 ng/ml) or histamine (1 h, 50 μM). The distribution of JAM-C and ZO-1 is shown. Double stained images were merged to analyze colocalization.