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. 2006 Nov 27;203(12):2703–2714. doi: 10.1084/jem.20051730

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Overexpression of mouse JAM-C reverses the increase of VE-cadherin–mediated adhesiveness caused by JAM-C knockdown. (A) The expression of mouse JAM-C and human JAM-C was analyzed by Western blot in HDMECs transfected with control siRNA (1), in HDMECs transfected with control siRNA, and with mouse JAM-C (2), in HDMECs transfected with siRNA against JAM-C (3), or in HDMECs transfected with siRNA against JAM-C and with mouse JAM-C (4) as indicated. (B) The adhesion of HDMECs transfected with control siRNA (open bars), of HDMECs transfected with control siRNA and with mouse JAM-C (shaded bars), of HDMECs transfected with siRNA against JAM-C (gray bars), or of HDMECs transfected with siRNA against JAM-C and with mouse JAM-C (hatched bars) to immobilized control Fc protein, Fc–VE-cadherin, or Fc–PECAM-1 is shown. Adhesion is shown as the percentage of adherent cells. Data are mean ± SD of a typical experiment; similar results were obtained in three separate experiments.