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. 2006 Nov 27;203(12):2691–2702. doi: 10.1084/jem.20061104

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — PDE5 inhibition reverts MDSC suppressive pathways. Splenic CD11b⁺ cells from C26GM tumor-bearing mice were isolated and added to CFSE-labeled splenocytes containing either (A) naive HA-specific CD8⁺ (CL4) cells or (B) naive HA-specific CD4⁺ (6.5) cells. The cultures were stimulated for 4 d with the relevant peptide in the presence or absence of 50 μg/ml sildenafil. Proliferation was evaluated as CFSE dilution by FACS analysis. (C) Splenic CD11b⁺ cells were magnetically purified from B16GM tumor-bearing C57BL/6-NOS^+/+ or C57BL/6-NOS^−/− mice and added to CFSE-labeled splenocytes containing naive OVA-specific CD4⁺ T cells. The cultures were stimulated for 4 d with the relevant peptide in the presence or absence of 50 μg/ml sildenafil. Proliferation was evaluated as CFSE dilution by flow cytometry. Data derived from one of two independent experiments with similar results are reported. Error bar values are shown.