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. 2006 Dec 25;203(13):2887–2893. doi: 10.1084/jem.20061519

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Copyright © 2006, The Rockefeller University Press

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Figure 4. — HIV-VSVG infects iDCs that express low levels of A3G. (A) iDCs were infected with HIV-VSVG and sorted after 5 d as GFP⁺ (infected) and GFP⁻ (noninfected) fractions. (B) RNA was extracted and subjected to RT-PCR for A3G (30 cycles), A3F (35 cycles), T5α (35 cycles), and tubulin. (C) Proteins extracted from the same cells were used in Western blot for A3G and tubulin. The figure shows two different cell preparations. NI, uninfected control cells.