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. 2006 Dec 25;203(13):2841–2852. doi: 10.1084/jem.20061884

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Inducible DC extensions in the terminal ileum. Explanted small intestinal segments from untreated (open bars) or noninvasive Salmonella-treated (filled bars) mice were analyzed by two-photon microscopy for the presence of trans-epithelial DC extensions. (A) The number of DC extensions was determined by inspection of each z-slice in image stacks collected in multiple sites within the indicated subregions of the small bowel. The data are presented as the average number of extensions ± SE/villus. The data show representative results from one of three independent experiments, each involving five random acquisitions per intestinal region with three to eight villi visualized per acquisition. (B) Average numbers of DC extensions in the proximal ileum of untreated (open bar) or antibiotic-treated (striped bar) mice were analyzed as in A. Results are representative of three separate experiments.