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. 2006 Dec 25;203(13):2841–2852. doi: 10.1084/jem.20061884

Figure 5.

Figure 5.

Characteristics of DC trans-epithelial extensions. (A) MHC CII-EGFP mice were deprived of food for 4 h before oral Salmonella administration. The terminal ileum was then imaged by intravital two-photon microscopy. The nuclei of all the cells were labeled with Hoechst 33342 (blue), and the epithelium was stained with SNARF (red). Two different DCs can be seen extending dendritic processes into the intestinal lumen. In this example, both BB (#) and finger-like (*) dendritic extensions can be seen in the same villus (also see Video S3). (B) MHC CII-EGFP mice were deprived of food for 4 h before oral noninvasive Salmonella administration. The terminal ileal epithelium was then labeled with SNARF-1 (red) and imaged by two-photon microscopy. Sequential images from a time series are shown at high magnifications and reveal a dendrite reaching the luminal side and acquiring the BB shape. The protrusion is almost completely retracted after 22 min (see Video S4).