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. 2006 Dec 25;203(13):2841–2852. doi: 10.1084/jem.20061884

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Copyright © 2006, The Rockefeller University Press

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Figure 7. — Interaction of inert particles and bacteria with DC extensions in the bowel lumen. (A) A time sequence of images from the same region of a villus in the terminal ileum of an MHC CII-EGFP mouse. The mouse was given noninvasive Salmonella (red) orally. A segment of the terminal ileum was isolated 5 h later, the epithelium was stained using Cell Tracker Blue, and the preparation was imaged by two-photon microscopy. A BB can be seen interacting with a fluorescent bacterium before internalizing it. (B) A three-dimensional section of a villus in the terminal ileum from an MHC CII-EGFP mouse. Noninvasive Salmonella (red) was given orally 5 h before the terminal ileum was explanted and stained with Cell Tracker Blue. A Salmonella bacterium just internalized by a DC extension with the typical BB shape is shown by the colocalization of green and red in the three-dimensional section (also see Videos S6 and S7).