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. 2006 Dec 25;203(13):2929–2937. doi: 10.1084/jem.20062206

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Copyright © 2006, The Rockefeller University Press

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Figure 3. — IL-21 expression. (A) Vα14 NKT cell–dependent IL-21 production. Liver MNCs were obtained after BCG injection (500 μg/mouse) and examined for IL-21 mRNA expression. (B) Identification of the source of IL-21. Vα14 NKT and conventional T cells were sorted from liver MNCs and examined for IL-21 mRNA expression. (C) Requirement of DCs for BCG-induced IL-21 expression by Vα14 NKT cells. Liver TCRβ⁺ cells were cultivated in the presence of 50 μg/ml BCG with (top) or without (bottom) BM-DCs for 24 h and analyzed for IL-21 mRNA expression. Liver TCRβ⁺ cells stimulated with 10 μg/ml anti-CD3 mAb were used as a positive (Pos.) control. (D) Requirement of IL-12– and CD1d-mediated signals for IL-21 mRNA expression upon BCG stimulation. An isotype control, anti–IL-12p40/p70, or anti-CD1d mAb (20 μg/ml) was added to the cultures of liver TCRβ⁺ cells and BM-DCs as described in C. All experiments were repeated twice with similar results.