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. 2006 Dec 25;203(13):2939–2951. doi: 10.1084/jem.20061552

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Splenomegaly caused by the loss of Bim is enhanced by concomitant Puma deficiency. Spleens from animals of the indicated genotypes were harvested. (a) Total cellularity determined by counting an aliquot of the single-cell suspensions using a hemocytometer and trypan blue exclusion. (b) Total cellularity of splenic T and B cells was determined by staining cell suspensions with fluorescence-conjugated antibodies specific for CD4 and CD8 or IgM and IgD, respectively. (c) The absolute numbers of activated CD44^highCD62L^low T cells were determined by staining splenic T cells with antibodies specific for CD4 or CD8 in combination with antibodies to CD44 and CD62L. (d and e) Absolute numbers of granulocytes (Mac-1⁺Gr-1⁺), macrophages (Mac-1⁺Gr-1⁻; d), and nucleated erythroid cells (Ter119⁺; e), were determined by flow cytometric analysis using fluorescence-conjugated antibodies. Bars represent means ± SEM (error bars) of 5–10 animals of each genotype.