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. 2006 Feb 20;203(2):281–287. doi: 10.1084/jem.20051637

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Copyright © 2006, The Rockefeller University Press

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Figure 2. — Impaired LAT phosphorylation and interaction with PLCγ1 and Grb-2 in TSAd-deficient T cells. LN T cells from TSAd (+/+) and (−/−) mice were stimulated with optimal concentrations of CD3 and CD28 mAb for the indicated times (in min). (A) PLCγ1 activation was assessed by Western blotting of whole cell lysates using a phospho-specific antibody. (B) LAT was immunoprecipitated from lysates. Phosphorylation of immunoprecipitated LAT on Y136 and Y195 and coimmunoprecipitation of PLCγ1 and Grb-2 was detected by Western blotting using specific antibodies. (C) Activation of ZAP-70 was assessed by Western blotting of whole cell lysates using a phospho-specific antibody.