Figure 2.

Increased CD4/CD8 ratio, augmented representations of activated T cells, and cytokine secretion by ex vivo crt^−/− T cells. (a) CD4/CD8 ratios in the spleen and lymph nodes. (b) Relative representations of CD69⁺ cells in CD4⁺ and CD8⁺ subsets (left) and CD25⁺ cells in CD4⁺ subset (right). Gray bars, crt^+/+ chimeras; black bars, crt^−/− chimeras. (c) Ex vivo cytokine secretion of CD4⁺ and CD8⁺ T cells. Intracellular staining for the indicated cytokine was performed on freshly isolated lymph node cells treated with PMA, ionomycin, and brefeldin A (see Materials and methods). This experiment is representative of six different mice per group.