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. 2006 Feb 20;203(2):461–471. doi: 10.1084/jem.20051519

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Copyright © 2006, The Rockefeller University Press

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Figure 5. — Augmented representation of effector–memory T cells in crt^−/− chimeras and in vitro responsiveness to suboptimal CD3ɛ stimulation. (a) Relative representations of CD44⁺CD62L⁻ cells in CD4⁺ and CD8⁺ subsets. Gray bars, crt ^+/+ chimeras; black bars, crt ^−/− chimeras. (b) CFSE dilution in purified CD4⁺ effector–memory cells stimulated with CD3ɛ mAb for 16 h (top) and CD3ɛ+CD28 mAbs for 40 h (bottom) as detailed in Materials and methods. The addition of a particular cytokine is indicated on top of the relative histograms. Numbers indicate the absolute values of cells recovered within the marker limits in standardized acquisitions. (c) Relative representations at different time points of effector–memory T cells with diluted CFSE (i.e., proliferating; top) and cells positive for annexin V (bottom) upon stimulation with the indicated mAbs for the indicated times. (d) IL-2 and IL-4 concentrations in culture supernatants of effector–memory T cells stimulated with the indicated mAbs for the indicated times. Gray bars, crt^+/+ chimeras; black bars, crt^−/− chimeras.