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. 2006 Feb 20;203(2):461–471. doi: 10.1084/jem.20051519

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Copyright © 2006, The Rockefeller University Press

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Figure 8. — Protracted NFAT activation and MAPK signaling in crt^−/− T cell clones. (a) Immunoblot analysis of NFAT1 in cytosolic and nuclear fractions of crt^+/+ and crt^−/− DO11.10tg T cell clones stimulated with CD3ɛ mAb for the indicated times. The experiment is representative of three different clones per group. (b) Immunoblot of NFAT1 in nuclear fractions of crt^+/+ and crt^−/− DO11.10tg T cell clones stimulated for 16 h with DCs loaded with decreasing doses of OVAp. The result is representative of three experiments with two different clones per group. (c) Immunoblot of phosphorylated p38MAPK, ERK, and c-jun in crt^+/+ and DO11.10tg T cell clones before and 16 h after crt^−/− stimulation. The result is representative of four different experiments with at least two different clones.