Figure 6.

Induction of Th1 and Tc1 responses by YF-17D is dependent on MyD88. Wild-type or MyD88^−/− mice were vaccinated subcutaneously with YF-17D. Control mice were injected with supernatants from mock transfected SW-480 cells. 10 d later, the draining lymph nodes were harvested and single cell suspensions were cultured in vitro in media alone or with a combination of CD8⁺ T and CD4⁺ T cell epitopes from YF-17D as described in Materials and methods. 3 d later, the induction of IFN-γ was measured by ELISA (A) and by intracellular cytokine staining (B and C). These data are representative of three independent experiments with three mice per group in each experiment.